G418是一種氨基糖苷類抗生素 ,在分子遺傳試驗(yàn)中，是穩(wěn)定轉(zhuǎn)染最常用的抗性篩選試劑。它通過抑制轉(zhuǎn)座子Tn601，Tn5 的基因,干擾核糖體功能而阻斷蛋白質(zhì)合成，對(duì)原核和真核等細(xì)胞產(chǎn)生毒素 ,包括細(xì)菌、酵母、植物和哺乳動(dòng)物細(xì)胞 ,也包括原生動(dòng)物和蠕蟲。當(dāng)neo基因被整合進(jìn)真核細(xì)胞DNA后 ,則能啟動(dòng)neo基因編碼的序列轉(zhuǎn)錄為mRNA ,從而獲得抗性產(chǎn)物氨基糖苷磷酸轉(zhuǎn)移酶的高效表達(dá) ,使細(xì)胞獲得抗性而能在含有G418的選擇性培養(yǎng)基中生長(zhǎng)。G418的這一選擇特性 ,已在基因轉(zhuǎn)移、基因敲除、抗性篩選以及轉(zhuǎn)基因動(dòng)物等方面得以廣泛應(yīng)用。
technical data:
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ITEM |
SPECIFICATION |
RESULT |
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Appearance |
White powder |
conforms |
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Solubility (Turbidity) 100mg/mL in H2O |
Clear to Very Slightly Hazy |
conforms |
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Biological Assay |
ED50 Resistant: ≥2500 ug/mL ED50 Sensitive: ≤400 ug/mL |
conforms |
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Absorbance: |
1mg/mL H2O:280nm < 0.015 100mg/Ml H2O: 570nm <0.10 |
conforms |
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pH |
4.6-6.0 |
5.6 |
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Heavy metals |
≤100 ppm |
90 ppm |
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Purity by HPLC
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≥90% |
94.97% |
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** (by Karl Fischer) |
< 10 % |
3.0 % |
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Potency (on a dry basis) |
Not less than 720 ug/mg |
740 ug/mg |
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CONCLUSION: THE RESULTS CONFORM WITH ENTERPRISE STANDARD
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